Contact Points
Date Quotation Due: All responses to this request for quote must be received by 3:00 PM Eastern Standard Time, on September 4, 2020 and must reference the request number cited above. Responses must be submitted electronically to the contact person cited above. Fax responses will not be accepted.
IMPORTANT: This is a request for quotations. If you are unable to quote, please so indicate in a reply email to the point of contact cited herein. This request does not commit the Government to pay any costs incurred in the preparation of the submission of a quotation or to contract for supplies or service. Supplies are of domestic origin unless otherwise indicated by quoter. Any representations and/or certifications attached to this Request for Quotation must be completed by the quoter.
Description of Requirement:
The National Center for Advancing Translation Sciences (NCATS) is in need of obtaining 100 ul packed cell volume/as well as media supernatants of multiple samples of human induced pluripotent stem cells (iPSCs), and iPSC derived Astrocytes to Cell Surface Proteomics for comprehensive analysis of cell surface proteins and secreted proteome profiling. Data from these two analyses will then be provided to the NCATS Stem Cell Translation Laboratory.
Independently and not as an agent of the Government, the Contractor shall furnish all the necessary services, qualified personnel, material, equipment, and facilities, not otherwise provided by the Government as needed to perform the Statement of Work below:
The vendor will perform cell surface proteomic analysis and secretome profiling of human iPSCs and iPSC-derived astrocytes to better understand changes regulating astrocytes reactivity.
Critical to success of this project are the capability to perform integrated proteomic and secretome profiling analysis for high resolution detection of proteins present at very low concentrations (micro molar levels) combined with identification of proteins expressed at cell surface.
The contractor will perform and/or provide the following:
- Sample preparation: For cell samples, we will extract the cell surface protein by using membrane protein extraction kit (Thermo). For cell culture media samples, we will process your samples according to our own SOP which including protein precipitation at first.
- Digestion: protein samples will be digested by trypsin.
- Peptide extraction and desalting: ziptips will be used in the desalting process in order to get the highest peptide recovery.
- Fractionation: For cell culture media samples, HPLC fractionation will be performed.
- Reverse-phase LC-MS/MS on the peptide mixture: Q Exactive™ HF MS/MS will be applied to analysis the peptide mixture; Q Exactive™ HF MS/MS is the newest version of MS with the highest resolution, compared with MALDI-TOF/TOF and other older version of LC-MS/MS.
- Database searching of the data from all LC-MS/MS experiments, quantification and posting/archiving the resulting data summary.
Government Responsibility:
SCTL, NCATS will provide different cell samples including human iPSCs, human iPSC-derived astrocyte-like cells as frozen cell pellets, as well as samples of the iPSC/iPSC astrocyte conditioned medium.
Delivery or Deliverables:
At the end of the project, a technical report will be provided listing the detailed experiment procedure, LC-MS/MS instrument parameters and all of the proteins identified & quantified information. Also, an excel file will be provided listing the detailed information related to the proteins identified and quantified,
Reporting Requirements:
The Contractor shall provide monthly updates on the progress of the project, or more often as appropriate.
Data Rights:
Data generated as a result of proteomics and secretome analysis will be the property of NIH, NCATS, SCTL.
Publications and Publicity:
The contractor will not publish any results of the work.
Confidentiality of Information:
The Contractor will keep the results of the contract confidential.
Quoter Terms and Conditions (To be completed by the Offeror):
- Period of Performance OR Delivery Date After Receipt of Order:
- Shipping Point (F.O.B. OR Destination):
- Payment Discount Terms:
- NIH BPA Number
- DUNS No:
- Name of Company:
- Street Address, City, State, Zip code:
- Name of Person Authorized to Provide Quote:
- Telephone Number and Email Address of Person Authorized to Provide Quote:
Contract Type:
A Fixed-Price type of Purchase Order award not exceeding the Simplified Acquisition Threshold is anticipated for any contract resulting from this requirement. The anticipated award date is September 11, 2020.
ADDITIONAL TERMS AND CONDITIONS, PROVISIONS, AND REPRESENTATIONS
The FAR Clauses are incorporated by reference and are provided in full text at https://www.acquisition.gov/
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- The provisions of FAR Clause 52.212-1 Instructions to Offerors—Commercial Items (OCT 2018) is applicable to this solicitation.
- The provisions of FAR Clause 52.212.2 Evaluation—Commercial Items (Oct 2014) is applicable to this solicitation. The Government will evaluate quotations or offers in accordance with FAR 13.106-2 and award a purchase order from this solicitation to the responsible offeror whose quote conforming to the solicitation will be most advantageous to the Government, price and other factors considered. The following factors shall be used to evaluate quotes:
- Technical capability of the item offered to meet the Government requirement;
- Price; and
- Past performance [see FAR 13.106-2(b)(3)].
- The provisions of FAR Clause 52.212-3, Offeror Representations and Certifications—Commercial Items (Dec 2019), is applicable to this solicitation.
- The provisions of FAR Clause 52.212-4, Contract Terms and Conditions—Commercial Items (OCT 2018) with Addenda: Stop Work Order, FAR Clause 52.242-15 (August 1989) is applicable to this solicitation.
- The provisions of FAR clause at 52.212-5, Contract Terms and Conditions Required to Implement Statutes or Executive Orders—Commercial Items (Aug 2020) is applicable to this solicitation:
52.222-3 Convict Labor (Jun 2003)
52.222-19 Child Labor-Cooperation With Authorities and Remedies (Jan 2020)
52.222-21 Prohibition of Segregated Facilities (Apr 2015)
52.222-26 Equal Opportunity (Sep 2016)
52.222-36 Equal Opportunity for Workers With Disabilities (Jun 2020)
52.222-50 Combating Trafficking in Persons (Jan 2019)
52.223-18 Encouraging Contractor Policies to Ban Text Messaging While Driving (Jun 2020)
52.232-33 Payment by Electronic Funds Transfer-- System for Award Management (Oct 2018)
In addition, the following FAR provisions are applicable to this solicitation and incorporated by reference: 52.204-7 System for Award Management (Oct 2018),
52.204-13 System for Award Management Maintenance (Oct 2018),
52.204-16 Commercial and Government Entity Code Reporting (Jul 2016),
52.204-18 Commercial and Government Entity Code Maintenance (Jul 2016).
The following provisions and clauses apply to this acquisition and are incorporated as an attachment. Offerors MUST complete the provision at 52.204-24 and submit a completed copy as a separate document with their quotation.
- FAR 52.204-24, Representation Regarding Certain Telecommunications and Video Surveillance Services or Equipment (Aug 2020)
- FAR 52.204-25, Prohibition on Contracting for Certain Telecommunications and Video Surveillance Services or Equipment (Aug 2020)
- FAR 52.204-26, Covered Telecommunications Equipment or Services-Representation (Dec 2019)
- NIH Invoice and Payment Provisions (2/2014)
SUMMARY STATEMENT:
Responses to this notice must include sufficient information to establish the interested parties’ bona-fide capabilities of providing the product or service. The response shall include: the unit price, list price, shipping and handling costs, delivery days after contract award, delivery terms, prompt payment discount terms, F.O.B. Point (Destination or Origin), product or catalog number(s); product description; and any other information or factors that may be considered in the award decision. Such factors may include: past performance; special features required for effective program performance; trade-in considerations; probable life of the item selected as compared with that of a comparable item; warranty considerations; maintenance availability; and environmental and energy efficiency considerations.
Interested parties may identify in writing their interest and capability in response to this requirement. Responses to this notice shall contain sufficient information to establish the interested parties’ bona-fide capabilities for fulfilling the requirement and include: unit price, list price, shipping and handling costs, the delivery period after contract award, the prompt payment discount terms, the F.O.B. Point (Destination or Origin), the Dun & Bradstreet Number (DUNS), the Taxpayer Identification Number (TIN), and the certification of business size. All offerors must have an active registration in the System for Award Management (SAM) www.sam.gov.
All responses must be received by closing date and must reference the announcement. Responses may be submitted electronically to the attention of the contract specialist. Fax responses will not be accepted.
All responsible sources may submit a bid, proposal, or quotation which shall be considered by the agency.