Contact Points
Pennsburg, PA 18073
simplified acquisition threshold are exempt from the requirements of FAR Part 6—Competition Requirements.
Hitech Instruments, Inc is the only authorized provider of this instrument at the NIH. Hitech Instruments is Zeiss’ exclusive distributor of this product at NIH and as such is the only authorized source to purchase from.
Hitech Instruments, Inc. has provided a sole source letter to the Government certifying that no other company is authorized distributor of Hitech Instruments, Inc. brand products.
Acquisition Authority: This acquisition is being conducted under Federal Acquisition Regulations (FAR) Part 13 – Simplified Acquisition Procedures, and the resultant contract award will include all applicable provisions and clauses in effect through Federal Acquisition Circular 2021-06 published in the Federal Register on July 12, 2021.
The Transcription Systems Dynamics and Biology Uni (TSDBU), Laboratory of Molecular Biology and Immunology (LMBI) with the National Institute on Aging requires the use of ZEISS Axio Vert.A1 FL-LED microscope system to allow for monitoring fluorescence in DAPI, GFP, TxRed, and Cy5 channels and capturing the fluorescence from the culture dishes. This microscope is suitable for quick checking the fluorescence regularly needed in the tissue culture room.
The transcription factor NF-κB regulates multiple aspects of innate and adaptive immune functions and serves as a pivotal mediator of inflammatory responses. To monitor the endogenous NF-κB signaling in innate immune cells and the role of non-coding genomic regions around NF-κB regulated genes, LMBI TSDBU has generated immortalized macrophages from CRISPR Knock-in fluorescent mouse model. LMBI TSDBU will generate mutants containing genomic deletions in different non-coding regions and monitor their effects on NF-κB signaling dynamics. For sorting the mutant cells, LMBI TSDBU will use fluorescent dCas9-gRNA RNP complex which needs to monitor regularly in the microscope to optimize the culture and sorting conditions. To regularly monitor the fluorescence of these immortalized wild-type and mutant cells, a fluorescence tissue culture microscope is needed in the tissue culture room.
Generic Name of Product: Fluorescence tissue culture microscope
- Purchase Description: ZEISS Axio Vert.A1 FL-LED is an entry-level research fluorescence tissue culture microscope including the following:
- On-site installation, training, and basic support
- One Preventative Maintenance visit one year after installation with full service exam and cleaning and all parts checked prior to warranty expiration.
- 12-month warranty.
Salient characteristics
The following salient characteristics are required:
- Upgradable to PlasDIC, DIC, iHMC, polarized light. Brightfield, Phase contrast, PlasDIC, improved Hoffman Modulation Contrast (iHMC) and Fluorescence contrast
- LED transmitted light; long lifetime of the light source.
- Single-channel LED fluorescence modules with
- adjustable intensity
- DAPI, GFP, TxRed, Cy5 Chroma ET filter sets
- Highest transmission
- Hard-sputtered, pixel-shift corrected
- Adjustable illumination
- No bulb changes/alignment
- No heating and cooling period required
- Compact build – no external light source
- 10x and 20x LD A-Plan phase contrast objectives
- Axiocam 304 mono camera
- Mini PC and 24" 1900x1200 monitor
Line item 1 - item number - HT-4310309040 Axiovert A1 TL/FL-LED = Qty 1
Line item 2 - item number - HT-4255239000 Binocular tube 45/23 = Qty 2
Line item 3 - item number HT-4251469031 Intermediate phototube, left = Qty 3
Line item 4 - item number - HT-4251469051 Slider with mirror 100% = Qty 4
Line item 5 - item number - HT-4440399000 Eyepiece Pl 10x/21 Br foc = Qty 5
The following provisions and clauses apply to this acquisition and are incorporated as an attachment. Offerors MUST complete the provision at 52.204-24 and 52.204-26 and submit completed copies as separate documents with their quotation.
1. FAR 52.204-24, Representation Regarding Certain Telecommunications and Video Surveillance Services or Equipment (Oct 2020)
2. FAR 52.204-25, Prohibition on Contracting for Certain Telecommunications and Video Surveillance Services or Equipment (Aug 2020)
3. FAR 52.204-26, Covered Telecommunications Equipment or Services-Representation (Oct 2020)
The Dun & Bradstreet Number (DUNS), the Taxpayer Identification Number (TIN), and the certification of business size must be included in the response.
All offerors must have an active registration in the System for Award Management (SAM) www.sam.gov.”
Quoter Terms and Conditions (To be completed by the Offeror):
1. Period of Performance OR Delivery Date After Receipt of Order:
2. Shipping Point (F.O.B. OR Destination):
3. Payment Discount Terms:
4. NIH BPA Number, if applicable:
5. DUNS No:
6. Name of Company:
7. Street Address, City, State, Zip code:
8. Name of Person Authorized to Provide Quote:
9. Telephone Number and Email Address of Person Authorized to Provide Quote:
Interested parties may identify in writing their interest and capability in response to this requirement. Responses to this notice shall contain sufficient information to establish the interested parties’ bona-fide capabilities for fulfilling the requirement and include: unit price, list price, shipping and handling costs, the delivery period after contract award, the prompt payment discount terms, the F.O.B. Point (Destination or Origin), the Dun & Bradstreet Number (DUNS), the Taxpayer Identification Number (TIN), and the certification of business size. All offerors must have an active registration in the System for Award Management (SAM) www.sam.gov.
All responses must be received by closing date and must reference the announcement. Responses may be submitted electronically to the attention of the contract specialist. Fax responses will not be accepted.
All responsible sources may submit a bid, proposal, or quotation which shall be considered by the agency.