NIH-NINDS-22-002751 - Notice of Intent

Submitted by patelsnv on
Post Date/ Solicitation Issue Date
Closing Response Date
Proposed Award Date
Project Title
Illumina NextSeq 500/500 High Output Sequencing Kits v2.5
Contracting Office
National Institute of Neurological Disorders and Stroke (NINDS)

Contact Points

Primary Contract Specialist

Valerie
Gregorio
valerie.gregorio@nih.gov
NAICS Code Number
334516
Analytical Laboratory Instrument Manufacturing
Small Business Size Standard
1000 employees
FPDS Classification Code
N/A
Estimated Period of Performance
N/A
Delivery of Goods
BLDG 10 RM 3B13
Set-Aside Status
Not Set Aside
Competition Status
Brand Name Only
Vendor Name
Illumina, Inc.
Address
5200 Illumina Way
San Diego, CA 92122-4616
United States
Single-Sole Source Determination
A more competitive product for NUPID lab research goals for ongoing project was not found during the market research phase. Even if other sequencing kits are available, the NextSeq kits are the only ones that would not jeopardize the results of ongoing research using valuable clinical samples. The lab requires NextSeq 500/550 High-Output Kits v2.5 (150 cycles) plus dual-indexing support on a high output run (up to 400 million reads) that’s sold by Illumina to allow for analysis of data from the single-nucleus RNAseq and Single-nucleus ATAC-seq libraries that are generated via the 10X Genomics Chromium Multiome Single-Cell Assay.
The efficiency of the High-Output chips allows for two main advantages:
(1) Sequencing of 6-8 samples at once using a single kit
(2) Achieving a high level of read-depth, which provides the best possible resolution when analyzing
the transcriptomic and epigenetic data.
The kits come with:
(1) High Output Reagent Cartridge
(2) High Output Flow Cell
(3) Buffer Cartridge

Illumina Inc. is the only vendor that provides these sequencing kits and do not have 3rd party vendors that provide the kits. Information on these products were provided by Andrew Eckert (aeckert@illumina.com, 858-232-4172), Illumina Inc. 5200 Illumina Way, San Diego, CA 92122-4616.
Background/Description of Requirement

The NUPID lab is performing a study to understand how the epigenetics and transcriptome of Vestibular Schwannomas that form in patients with NF2 can shed light on their tumorigenesis and sustained growth. Because the 10X Multiome assay generates two libraries per sample, one containing the transcriptomic information encoded by captured mRNA, and the other containing epigenetic information held by captured DNA fragments, the experiments require more sequencing kits so that analysis of both indicies at once can be done. The high-output kits allow us to sequence between 6-8 samples at once, which is far more efficient than what would be allowed using the medium-output variant of the same kit.

 

Interested parties may identify in writing their interest and capability in response to this requirement. Responses to this notice shall contain sufficient information to establish the interested parties’ bona-fide capabilities for fulfilling the requirement and include: unit price, list price, shipping and handling costs, the delivery period after contract award, the prompt payment discount terms, the F.O.B. Point (Destination or Origin), the Dun & Bradstreet Number (DUNS), the Taxpayer Identification Number (TIN), and the certification of business size. All offerors must have an active registration in the System for Award Management (SAM) www.sam.gov.

All responses must be received by closing date and must reference the announcement. Responses may be submitted electronically to the attention of the contract specialist. Fax responses will not be accepted.

All responsible sources may submit a bid, proposal, or quotation which shall be considered by the agency.