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The CBBU at NINDS studies microtubules(MTs) and microtubule associated proteins(MAPs) and their interactions. Tubulin is one of the most prevalent proteins in the brain and the polymerized form, microtubule’s are essential for a wide variety of cellular phenomena. Perturbation to the microtubule cytoskeleton can lead to sever degrative disease. The construction and disassembly of microtubules is a very regulated process in cells. To understand the fundamental principles we apply label free darkfield imaging to microtubule sample in vitro. The acquisition on an enhanced darkfield condenser and liquid light guide will allow us to study this process at the single protofilament level and yield insights not currently achievable with existing equipment.
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