NIH-NINDS - 22-011794 - Notice of Intent

Submitted by patelsnv on Fri, 07/29/2022 - 10:00
Post Date/ Solicitation Issue Date
Closing Response Date
Proposed Award Date
Project Title
Knockout Cell Line
Contracting Office
National Institute of Neurological Disorders and Stroke (NINDS)

Contact Points

Primary Contract Specialist

NAICS Code Number
Analytical Laboratory Instrument Manufacturing
Small Business Size Standard
1,000 employees
FPDS Classification Code
Estimated Period of Performance
Delivery of Goods
BLDG 35A RM 3D911
Set-Aside Status
Not Set Aside
Competition Status
Brand Name Only
Vendor Name
3696 Haven Ave, Suite A
Redwood City, CA 94063
United States
Single-Sole Source Determination
Vedrana Mikusevic entered a request for information and quotes into CREX, the collaborative research exchange for the NIH
intramural research program. She inquired about the engineering of an immortalized cell line with a key protein knocked out.This
cell line will be used in the Mindell lab to to study CLC7 and lysosomal pH regulation. She discussed the design of the cells,
timeline for production and cost with numerous vendors. She narrowed down the options based on what was discussed and then
obtained quotes from Synthego, HD Biosciences and SystemBio.
Background/Description of Requirement

Our goal in the Mindell lab is to better understand lysosomal pH regulation. The signaling lipid PI(3,5)P2 modulates lysosomal dynamics, including by regulating lysosomal ion channels, raising the possibility that it could contribute to lysosomal pH regulation. We have demonstrated that depleting PI(3,5)P2 by inhibiting the kinase PIKfyve causes lysosomal hyperacidification, primarily via an effect on ClC-7. We have also found that PI(3,5)P2 directly inhibits ClC-7 transport and that this inhibition is eliminated in a disease-causing gain-of-function ClC-7 mutation. Together, these observations suggest an intimate role for ClC-7 in lysosomal pH regulation.These two immortalized cell lines will allow us to evaluate the effect of this lysosomal regulating lipid (PI35P2) on the chloride/proton antiporter CLC-7 by deleting an enzyme responsible for regulating it.

Interested parties may identify in writing their interest and capability in response to this requirement. Responses to this notice shall contain sufficient information to establish the interested parties’ bona-fide capabilities for fulfilling the requirement and include: unit price, list price, shipping and handling costs, the delivery period after contract award, the prompt payment discount terms, the F.O.B. Point (Destination or Origin), the Dun & Bradstreet Number (DUNS), the Taxpayer Identification Number (TIN), and the certification of business size. All offerors must have an active registration in the System for Award Management (SAM)

All responses must be received by closing date and must reference the announcement. Responses may be submitted electronically to the attention of the contract specialist. Fax responses will not be accepted.

All responsible sources may submit a bid, proposal, or quotation which shall be considered by the agency.