Contact Points
1. Spatial RNA profiling and quantification of >18000 RNAs in a single reaction
2. Able to quantify individual RNA and proteins and provide digital output
3. Visualization of high-resolution morphological detail of source tissue using fluorescence microscopy
4. Dynamic range of ~5.5 logs (base 10)
5. Compatibility with FFPE and FF tissue sections
6. Non-destructive to tissue samples
7. Inclusion of 6 regions per slide (patient) for intrapatient statistical comparison
the GeoMx DSP platform measures local protein levels, and can
be combined with RNA expression, within heterogeneous tissue
samples. Combining both multiplexed nucleic acid and protein
on the same platform gives researchers the ability to spatially
resolve RNA when suitable antibodies do not exist. The GeoMx
DSP assay is performed on the GeoMx DSP platform which
includes imaging and fluidic components to capture spatial
context, and current nCounter® or next-generation sequencing
(NGS) instruments provide the quantification.
Glioblastomas are the most common malignant CNS neoplasm. Despite aggressive surgery, chemortherapy and brain radiation, median survival is only 15 months and the disease remains uniformly fatal. The NOU performs mission critical research on the molecular and cellular biology unique to glioblastomas with an expressed goal of developing novel therapeutic strategies to help patients. We recently used a publicly available genetic glioma dataset to probe a novel hypothesis regarding how primary cilia may affect glioma signal transduction and cancer phenotype. The data was confirmed using a separate, unrelated, publicly dataset. The goal of this purchase through GeoMx is to use tissues from our patients available in the biobank within the Surgical Neurology Branch (SNB) to confirm that our findings are indeed relevant to the patient population here at NIH whose lives we seek to improve. This is a necessary and important step in order to publish our findings in a high-impact journal. Additionally, the information gained will allow us to leverage our limited resources to determine and develop the targets with highest success potential.
Interested parties may identify in writing their interest and capability in response to this requirement. Responses to this notice shall contain sufficient information to establish the interested parties’ bona-fide capabilities for fulfilling the requirement and include: unit price, list price, shipping and handling costs, the delivery period after contract award, the prompt payment discount terms, the F.O.B. Point (Destination or Origin), the Dun & Bradstreet Number (DUNS), the Taxpayer Identification Number (TIN), and the certification of business size. All offerors must have an active registration in the System for Award Management (SAM) www.sam.gov.
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