Contact Points
Bruker supports this application with the objective Z-piezo stage, a fast piezoelectric objective positioner. Synchronizing resonant galvo imaging to Z-piezo steps in Bruker’s Prairie View acquisition software supports sufficient speeds for volume scanning.
In the exemplary volume scanning data featured here, four 441 × 512-pixel imaging planes were separated by 20 μm and imaged sequentially at a stack rate of 5 Hz in an imaging session. Even faster rates can be achieved with other settings.
To take advantage of multiphoton technology, which allows imaging down to 1mm within a sample, Bruker offers 0.25 mm, 0.4 mm, and 1 mm with 0.05 μm step size working distance Z-piezo stages. The piezo can be used with variety of objectives offered commercially.
The operation of the piezo is controlled in Bruker’s proprietary software, Prairie View. With this system, acquisition that is calibrated, fast, and bidirectional is supported. Additional time between frames can be set to give the Z-piezo stage time to settle before the acquisition of the subsequent frame. To improve positioning accuracy during data acquisition, a calibration procedure can be performed at a single click.
The objective Z-piezo stage is an add-on module that consists of a piezo stage, a set of objective adapters, and an electronics control box. The module can be installed easily by the user. Additionally, remote support or an onsite visit is provided.
The unique features of Objective Z-Piezo Stage from Bruker Nano, Inc. will allow the lab to take synchronizing resonant galvo imaging to Z-piezo steps in Bruker’s Prairie View acquisition software supports sufficient speeds for volume scanning on mice cortex in vivo respectively.
- This acquisition is restricted to Bruker Nano, Inc.
- Because of the urgency of this requirement, only one source will be solicited
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