Contact Points
• Spectral range is from 350-650 for the SB variant
• must have a good match for opsins, FL proteins between BFP to iRFP, and calcium indicators such as GCaMP6 or RCaMP
• 20 microseconds switching speed
To study the neuro circuits in the animal brains, we need to record the whole cell current in the brain slices, especially from the acute brain slices. So we need to approach the optogenetic stimulation on the specific regions and manipulate the activation or inhibitory of subpopulation neurons. Thus we need pE300-Ultra, which system offers precise control over wavelength irradiance and shuttering. To precisely manipulate the neuronal activation, we need the system which could offer The pE-300ultra offers triggering from multiple TTL inputs which, coupled with the ability to mount inline excitation filters, provides microsecond switching of pre-filtered excitation light. When paired with today’s high performance multi-band filter sets, this facilitates imaging traditionally achieved using a white light source and a filter wheel, with all the benefits of LEDs – and most excitingly at speeds not previously so affordable.
Item intended to acquire:
| Catalog Number | Quantity | Description | Manufacturer |
| OCL-PE300UTLSB40 | 2 | pE-300 Ultra LED,LLG "ready" single band / UV @ 365 system, with adapter and power cord. | Evident Scientific |
| OCL-PE1906 | 2 | Liquid Light Guide 1.5m, 3mm dia for pE-800, pE-300 lite/white/ultra LLG & pE-4000 | Evident Scientific |
| 5-UL604 | 2 | U-LLGAD; collimating light Guide Adapter for U-HGLGPS | Evident Scientific |
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