Contact Points
- Statement of Need and Purpose:
The Cellular Neurophysiology Section’s research studies show that SNc dopamine neurons are differentiated from other less vulnerable neurons based on their intracellular calcium dynamics. Because of this, the Cellular Neurophysiology Section designs experiments to compare calcium dynamics between different dopamine neuron subpopulations. To perform these experiments, the Section requires a high-resolution, high-sensitivity, digital CMOS camera that is brand name or equal to Hamamatsu’s ORCA-Fusion BT digital CMOS camera, to perform high resolution, low light imaging of either calcium-sensitive or voltage-sensitive dyes or proteins. The Section also requires a CoaXpress board and cable (or equal high-speed interface cable) to enable fast transfer between the ORCA-Fusion camera and computer.
- Background Information and Objective:
The Cellular Neurophysiology Section of the National Institute of Neurological Disorders and Stroke (NINDS) performs research focusing on circuit and cellular physiology of dopaminergic neurons located in the midbrain. In particular, the Section seeks to understand excitability and firing patterns of dopaminergic neurons located in the substantia nigra pars compacta (SNc), neurons that undergo selective cell death in Parkinson’s disease patients.
- Generic Name of Product:
High resolution, high sensitivity digital scientific CMOS camera
- Brand Name or Equal to:
- Manufacturer Name: Hamamatsu
- Product Brand Name: ORCA-Fusion BT digital CMOS camera
- Manufacturer Model/Type No.: C15440-20UP
- Salient Characteristics:
- Resolution: 2304 (H) x 2304 (V) pixels
- Cell size: 6.5 μm (H) × 6.5 μm (V)
- Readout speed: 89 frames per second (at 2304 x 2304 pixels, 16-bit)
- Readout noise: 0.7 electrons rms
- Lightsheet readout mode
- Capable of time-lapse live cell imaging by low light excitation
- Dynamic range: 21,400:1
- Quantum Efficiency: 95% at 550 nm
- A/D converter: 16-bit, 12-bit, 8-bit
- CoaXPress board and cable or equal included
- USB 3.0 card included
- USB3.0A-microB cable, power supply cable, and trigger cable included
- HCImage Live software or equal included
- C-mount full height mounting bracket included
- Quantity: One (1)
7. Delivery Terms:
The Contractor shall deliver the above-described equipment within sixty (60) days after receipt of order. The equipment shall be delivered to the National Institutes of Health, 35 Convent Drive, Building 35A, Room 3C-1002, MSC 3700, Bethesda, Maryland 20892, between the hours of 8:00am and 5:00pm local prevailing time, Monday through Friday. Delivery shall be Freight on Board (F.O.B.) destination and include inside delivery.
- Period of Performance:
Not applicable.
- Options:
Not applicable.
- Other Considerations:
The Contractor shall warrant the above-described equipment against defects in material and workmanship for a minimum of twelve (12) months from date of equipment delivery and acceptance.
Interested parties may identify in writing their interest and capability in response to this requirement. Responses to this notice shall contain sufficient information to establish the interested parties’ bona-fide capabilities for fulfilling the requirement and include: unit price, list price, shipping and handling costs, the delivery period after contract award, the prompt payment discount terms, the F.O.B. Point (Destination or Origin), the Dun & Bradstreet Number (DUNS), the Taxpayer Identification Number (TIN), and the certification of business size. All offerors must have an active registration in the System for Award Management (SAM) www.sam.gov.
All responses must be received by closing date and must reference the announcement. Responses may be submitted electronically to the attention of the contract specialist. Fax responses will not be accepted.
All responsible sources may submit a bid, proposal, or quotation which shall be considered by the agency.