NINDS-NOI-20-008401

1. Statement of Need and Purpose:
   The Membrane Transport Biophysics Section (MTBS) of the National Institute of Neurological Disorders and Stroke (NINDS) requires laser diode modules of different wavelengths, a 532nm diode-pumped solid-state (DPSS) laser and electro-mechanical shutter to illuminate fluorescent objects when performing single molecule imaging with a total internal reflection fluorescence (TIRF) microscope (Equipment).

2. Background Information and Objective: 
   The Membrane Transport Biophysics Section (MTBS) performs experiments examining the function of lysosomes from acidification and ion fluxes to cell metabolism and amino acid equilibrium. Lysosomes play a key role in the metabolism of the cell and have been implicated in numerous lysosomal storage disorders as well as broader systemic diseases. The MTBS requires this Equipment to image protein dynamics at the single molecule level.  The MTBS will use the Equipment to stimulate  fluorophores during microscope imaging.

3. Generic Name of Product(s):  
   1. One 445 nm, 100mW blue laser diode module - laser head, controller, 100-240V AC external power supply, and cables included
   2. One 488 nm, 100mW blue laser diode module - laser head, controller, 100-240V AC external power supply, and cables included
   3. One 532 nm, 100mW green laser module - laser head, controller, heatsink, 100-240V AC external power supply, and cables included
   4. One electro-mechanical shutter for 532 nm, 100mW laser module - laser head, controller, 100-240V AC external power supply, and cables included
   5. One 642 nm, 130mW red laser diode module - laser head, controller, 100-240V AC external power supply, and cables included
   6. One 730 nm, 40mW infrared (IR) laser diode module - laser head, controller, 100-240V AC external power supply, and cables included

4. Product Description: 

The above listed Equipment must be brand name or equal to the following:

 i.   Manufacturer: Oxxius

      Manufacturer Part Number: LBX-445

      Item Description: LBX series laser diode (445 nm wavelength)

 ii.  Manufacturer: Oxxius

      Manufacturer Part Number: LBX-488

      Item Description; LBX series laser diode (488 nm wavelength)

iii.  Manufacturer: Oxxius

      Manufacturer Part Number: LCX-532L

      Item Description: LCX series DPSS laser (532 nm wavelength)

iv.  Manufacturer: Oxxius

      Manufacturer Part Number: ACX-SHTE

      Item Description: electro-mechanical shutter for Oxxius LCX-532L DPSS laser

v.   Manufacturer: Oxxius

      Manufacturer Part Number: LBX-642

      Item Description: LBX series laser diode (642 nm wavelength)

vi.  Manufacturer: Oxxius

      Manufacturer Part Number: LBX-730

      Item Description: LBX series laser diode (730 nm wavelength)

5. Salient Characteristics:

      i. Oxxius LBX-445 laser diode

• Includes laser head, controller, external 100-240V AC power supply, and cables
• Emission wavelength: 445 nm
• Wavelength range: ± 5 nm
• Output power: 100mW
• Power adjustment range: 0 – 100%
• Optical noise: ≤ 0.2% RMS
• TEM00 Beam
• Beam divergence: ≤ 1.2 mrad
• Control mode: automatic power control, automatic current control

      ii. Oxxius LBX-488 laser diode

• Includes laser head, controller, external 100-240V AC power supply, and cables
• Emission wavelength: 488 nm
• Wavelength range: ± 5 nm
• Output power: 100mW
• Power adjustment range: 0 – 100%
• Optical noise: ≤ 0.2% RMS
• TEM00 Beam
• Beam divergence: ≤ 1.2 mrad
• Control mode: automatic power control, automatic current control   

      iii. Oxxius LCX-532L DPSS laser

• Includes laser head, controller, heatsink, external 100-240V AC power supply, and cables
• Emission wavelength: 532.3 nm
• Wavelength range: ± 5 nm
• Output power: 100mW
• Power adjustment range: L1C-MPA: 0 - 100%
• Optical noise: ≤ 0.2% RMS
• TEM00 Beam
• Beam divergence: 1.0 to ± 0.2 mrad
• Control mode: automatic power control

      iv. Oxxius ACX-SHTE electro-mechanical shutter

• Actuated via LCX-532L embedded software
• Direct mount on Oxxius LCX-532L DPSS laser

      v. Oxxius LBX-642 laser diode

• Includes laser head, controller, external 100-240V AC power supply, and cables
• Emission wavelength: 642 nm
• Wavelength range: -7/+5 nm
• Output power: 140mW
• Power adjustment range: 0 – 100%
• Optical noise: ≤ 0.2% RMS
• TEM00 Beam
• Beam divergence: ≤ 1.3 mrad
• Control mode: automatic power control, automatic current control

      vi. Oxxius LBX-730 laser diode

• Includes laser head, controller, external 100-240V AC power supply, and cables
• Emission wavelength: 730 nm
• Wavelength range: ± 10 nm
• Output power: 40mW
• Power adjustment range: 0 – 100%
• Optical noise: ≤ 0.5% RMS
• TEM00 Beam
• Beam divergence: ≤ 1.7 mrad
• Control mode: automatic power control, automatic current control

6. Quantity: 

          One (1) each of Items i. through vi. described herein.

7. Delivery Terms: 
   The Contractor shall deliver the Equipment Items described herein within sixty (60) days after receipt of order. The Equipment shall be delivered between the hours of 8:00am and 5:00pm Eastern Time, Monday through Friday, to:

          Attn: Dr. Joseph Mindell

          National Institutes of Health - Main Campus

          35 Convent Drive

          Building 35A, Room 3D913

          Bethesda, MD 20892

          Delivery must be Freight on Board (F.O.B.) destination including inside delivery.

8. Other Considerations:

All Equipment shall be warranted to be free from material or workmanship defects for a period of one (1) year from the date of delivery and acceptance by the Government.  All transportation, insurance and shipping charges associated with warranty service and repairs of the Equipment shall be paid by the Contractor, and all efforts will be made to minimize downtime of Equipment operation due to malfunction.   Repaired or replacement Equipment parts shall be warranted for one (1) year from the date of repair or delivery and acceptance.