Vendor Address
7068 Koll Center Parkway
Suite 401
Pleasanton, CA 94566
Rationale/ Salient Characteristics
The Drop-Seq technique is a newly developed and patented technique by which a flow of beads suspended in lysis buffer and a flow of a single-cell suspension are brought together in a microfluidic chip that generates nanoliter-sized emulsion droplets, which are then placed on a microfluidics chip and analyzed by the Chromium Controller machine. The Controller machine already exists and is available for our use. This machine allows for the high-throughput and rapid processing of the sample. It will process only 10X Genomics microfluidic chips, which cannot be obtained from any other vendor.
Single-Sole Source Determination
Market research efforts included attending scientific meetings where single-cell transcriptome analysis was the topic of presentations and consultation with the NIMH/NHGRI Microarray Core Director. One such meeting, at the National Academy of Sciences on development of biomarkers for neurosciences, included a presentation by the developer of the technique (http://dx.doi.org/10.1016/j.cell.2015.05.002). A Medline literature search was performed to find alternative ways to achieve our goals in cost- and time-effective manners. Reviews comparing methods (https://www.ncbi.nlm.nih.gov/pubmed/28091601; http://dx.doi.org/10.1016/j.molcel.2017.01.023), and it concluded that the 10X Genomics “Drop-Seq” single cell analysis method we selected was the most cost-effective and appropriate approach because it permits analysis of single-cell gene expressionin large numbers of cells with adequate genome coverage. A leading expert in gene expression analysis methods, advised me that this is the preferred method, and he has already procured some of the reagents for the analysis.
Background/Description of Requirement
Cat #1000269: (3) Chromium Single Cell 3.1' GEM, Library & Gel Bead Kits v3, 4 rxns
Cat #1000127: Chromium Chip G Single Cell Kit, 16 rxns
Cat #1000120: Chromium Chip G Single Cell Kit, 48 rxns
The Section on Functional Neuroanatomy does research on the cellular and molecular interactions between the immune system and the brain to elucidate how neuroimmune communication contributes to affective states in animals. Understanding of the bases for communication could lead to novel targets and treatments for affective disorders in humans. Our work has shown that the neurovasculature is altered in chronically stressed mice. We seek to understand whether similar dysfunction occurs in humans with major depression.
