22-006018

Submitted by fratecm on Sun, 07/24/2022 - 12:16
Post Date/ Solicitation Issue Date
Closing Response Date
Proposed Award Date
Project Title
RNA in situ hybridization probes and reagent kits
Contracting Office
National Institute of Mental Health (NIMH)

Contact Points

Primary Contract Specialist

Cheryl
Leone
cleone@mail.nih.gov

Secondary Contracting Officer

Dionne
Draper
cleone@mail.nih.gov
NAICS Code Number
325998
All Other Miscellaneous Chemical Product and Preparation Manufacturing
FPDS Classification Code
6810
Estimated Period of Performance
n/a
Delivery of Goods
Within 1 month of award being issued
Competition Status
Brand Name Only
Vendor Name
ACDBio
Vendor Address
7707 Gateway Blvd.
Newark, CA 94560
Vendor Name
ACDBio
Address
7707 Gateway Blvd.
Newark, CA 94560
Single-Sole Source Determination
One major research project of the Hattar Lab is to determine the transcription profiles of cell types involved in Intrinsically photosensitive retinal ganglion cells (ipRGCs)-brain neural circuits. We, and many other labs, have been using probes and reagents from RNAscope Product line to perform systematic RNA in situ hybridization experiments to visualize the RNA localization within cells. Since ACD Bio-techne is the sole supplier for RNAscope Product line, this is a sole source procurement.

I hereby determine that the RNAScope in situ hybridization probes and reagent kits listed herein is available from only one source, and competition is precluded for the reasons indicated below. There are no substitutes available.
Background/Description of Requirement
      1. Salient characteristics
    1. RNAscope® is a novel proprietary RNA in situ hybridization (ISH) method that enables routine detection and visualization of virtually any expressed gene in any tissue such as FFPE, Fresh frozen and fixed-frozen tissue.
    2. RNAscope® was designed to amplify target-specific signal without also amplifying the background signal, resulting in marked improvement in signal-to-noise ratio.This is accomplished by ACD’s patented double Z-probe design for in situ hybridization, where two independent probes (a double Z probe pair) are required to hybridize in tandem to the target sequence in order for signal amplification to occur. Since it is highly unlikely that two independent probes will hybridize to a nonspecific target right next to each other, this design concept ensures highly selective amplification of target-specific signals, therefore improving both sensitivity and specificity.
    3. Based on its patented probe design, RNAscope enables as much as 400-fold improvement over conventional RNA ISH, resulting in high detection sensitivity.
    4. Technology enables in situ detection of RNA molecules as small as 300bp, including degraded mRNA.
    5. ISH nature enables preservation of cellular and molecular context

Interested parties may identify in writing their interest and capability in response to this requirement. Responses to this notice shall contain sufficient information to establish the interested parties’ bona-fide capabilities for fulfilling the requirement and include: unit price, list price, shipping and handling costs, the delivery period after contract award, the prompt payment discount terms, the F.O.B. Point (Destination or Origin), the Dun & Bradstreet Number (DUNS), the Taxpayer Identification Number (TIN), and the certification of business size. All offerors must have an active registration in the System for Award Management (SAM) www.sam.gov.

All responses must be received by closing date and must reference the announcement. Responses may be submitted electronically to the attention of the contract specialist. Fax responses will not be accepted.

All responsible sources may submit a bid, proposal, or quotation which shall be considered by the agency.