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Purchase Description: (16) mTeSR plus, (8) STEMdiff Hematopoietic Kit, (8) STEMdiff Microglia Differentiation Kit, (4) STEMdiff Microglia Maturation Kit
5. Salient characteristics
• mTeSR Plus: cGMP, enhanced buffering and stabilized FGF2
• STEMdiff Hematopoietic Kit: for differentiation of human iPS cells into hematopoietic progenitor cells, facilitates high efficiency generation of CD34+, CD45+, and CD43+ cells in serum-free and feeder-free conditions
• STEMdiff Microglia Differentiation Kit: for differentiation of iPS-derived hematopoietic progenitors into microglia precursors; greater than 90% of source iHPC differentiate into microglia, cells demonstrate functional behaviors characteristic of microglia
• STEMdiff Microglia Maturation Kit: for maturation of microglia precursors derived from iHPC
Building F
Kent, Washington 98032
To specifically study microglia, there are multiple stages of the differentiation process. The iPSC will first be differentiated to a hematopoietic progenitor cell, which is similar to a hematopoietic stem cell; a cell type capable of myelopoiesis. This first stage requires the iHPC media kit, and is comprised of multiple stages itself. The second step of differentiation is the microglial differentiation, a process which is divided amongst two separate kits: the iMGL Differentiation kit and the iMGL maturation kit. Upon terminal differentiation of these cells, we complete several functional assays to compare across the DSWR group, DSRD group, and KN control group.
To specifically study microglia, there are multiple stages of the differentiation process. The iPSC will first be differentiated to a hematopoietic progenitor cell, which is similar to a hematopoietic stem cell; a cell type capable of myelopoiesis. This first stage requires the iHPC media kit, and is comprised of multiple stages itself. The second step of differentiation is the microglial differentiation, a process which is divided amongst two separate kits: the iMGL Differentiation kit and the iMGL maturation kit. Upon terminal differentiation of these cells, we complete several functional assays to compare across the DSWR group, DSRD group, and KN control group.
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