Contact Points
Secondary Contracting Officer
Suite 130
Madison, WI 53711-1067
The purpose of the requirement is to provide derived neurons and supplements for cytotoxicity measurement. The Therapeutic Development Branch (TBD) within the National Center for Advancing Translational Sciences (NCATS) Division of Preclinical Innovation (DPI) collaborative project under HEAL Initiative is aimed at developing assays for predictive toxicity of siRNA or antisense oligonucleotides (ASOs). 2D cultures using human induced pluripotent stem cell (hiPSC)-derived neurons (from non-fetal tissue source) have been adapted and extensively optimized by NCATS scientists to successfully predict neurotoxicity of ASOs.
2D ihPSC-derived spinal motor neurons co-cultured with astrocytes allows cellular viability and cytotoxicity measurement over the course of the culture. Changes in viability following siRNA or ASO treatment can be quantified to determine cytotoxicity and is typically used as a secondary screen for hits detected with 3D cultures.
Previous optimization of both neuronal 2D cultures have established the optimal number of neurons for both types of cultures to screen ASOs for cytotoxicity and have been used to successful complete early Milestone of the ongoing this project. In order to complete the HEAL collaborative project hiPSC neurons and supplements for 2D culture plates are needed and is a requirement to meet the goals of this project.
Generic Name of Product:
Cryopreserved human iPSC derived neurons and supplements
Purchase Description:
Trade name: Human Spinal Motor Neurons
Manufacture: BrainXell
Model: Derived from iPSC line WC-30, male,
1 Vial of ≥ 5 x 10^6 viable cryopreserved cells
Includes BrainFast Motor, SK, and D4 Supplements
Lot # 230323A
Trade name: Human Spinal Motor Neurons (GFP)
Manufacture: BrainXell
Model: Derived from iPSC line WC-30-GFP, male, Ubiquitously expressing eGFP
1 Vial of ≥ 2 x 10^6 viable cryopreserved cells.
Includes BrainFast Motor, SK, and D4 Supplements.
Lot # 230725C
Domestic Overnight Shipping: Small Liquid Nitrogen Vapor Shipper
Includes return of vapor shipper to BrainXell.
Salient characteristics:
iPSCs that are derived from the same patient as cells used in other assays reduces the impact of genetic diversity on assay variability.
Quantity:
(10) Human Spinal Motor Neurons, Lot # 230323A
(5) Human Spinal Motor Neurons (GFP), Lot #230725C
Interested parties may identify in writing their interest and capability in response to this requirement. Responses to this notice shall contain sufficient information to establish the interested parties’ bona-fide capabilities for fulfilling the requirement and include: unit price, list price, shipping and handling costs, the delivery period after contract award, the prompt payment discount terms, the F.O.B. Point (Destination or Origin), the Dun & Bradstreet Number (DUNS), the Taxpayer Identification Number (TIN), and the certification of business size. All offerors must have an active registration in the System for Award Management (SAM) www.sam.gov.
All responses must be received by closing date and must reference the announcement. Responses may be submitted electronically to the attention of the contract specialist. Fax responses will not be accepted.
All responsible sources may submit a bid, proposal, or quotation which shall be considered by the agency.