DA-20-005544

Background Information and Objective: 

The SCTL requires the acquisition of one (1) x KAPA mRNA Capture Kit only (96rxn), two (2) x KAPA Pure Beads 60ml, and four (4) x KAPA mRNA Hyper Prep (96rxn), and one (1) x KAPA RNA Hyper+RiboErase HMR (96rxn) to enable diverse library preparation methods for a variety of sample types and application needs. 

The KAPA mRNA HyperPrep Kit utilizes novel chemistry that enables the combination of enzymatic steps and fewer reaction purifications, resulting in a truly streamlined solution for the preparation of high-quality RNA-seq libraries. The strand-specific workflow is flexible—supporting library construction from lower-input amounts and degraded samples and mRNA capture. Kits contain all reagents required for RNA enrichment (if performed) and library preparation. Reaction buffers are supplied in convenient formats comprising all of the required reaction components. This minimizes the risk of RNase contamination, ensures consistent and homogenous reaction composition, and improves uniformity among replicate samples. Similarly, a single enzyme mixture is provided for each step of the library construction process, reducing the number of pipetting steps. The KAPA RNA HyperPrep Kit with RiboErase (HMR) for Illumina sequencing contains all of the buffers and enzymes required for depletion of ribosomal RNA (rRNA) and the rapid construction of stranded RNA-Seq libraries from 25 ng – 1 µg of purified total RNA. The procedures are automated in SCTL lab existing liquid handler, the protocols are provided and validated by Roche automation team. The KAPA mRNA Capture Kit only (96rxn), and KAPA Pure Beads 60ml are required for reagent overage volume on automation. 

Generic Name of Product:
1.    KAPA mRNA Capture Kit only (96rxn)
2.    KAPA Pure Beads 60ml
3.    KAPA mRNA Hyper Prep (96rxn)
4.    KAPA RNA Hyper+RiboErase HMR (96rxn)

Purchase Description:
1.    KAPA mRNA Capture Kit only (96rxn) (cat. 07962240001)
2.    KAPA Pure Beads 60ml (cat. 07983298001)
3.    KAPA mRNA Hyper Prep (96rxn) (cat. 08098123702)
4.    KAPA RNA Hyper+RiboErase HMR (96rxn) (cat. 08098140702)

Salient Characteristics:
Item #1: KAPA mRNA Capture Kit only (96rxn) (Cat. No. 07962240001)
The kit contain mRNA capture beads and wash buffer required for mRNA enrichment before library preparation. 
Item #2: KAPA Pure Beads 60ml (Cat. No. 07983298001)
KAPA Pure Beads offer a tunable and highly consistent solution for reaction purification and size selection in DNA and RNA next-generation sequencing library construction workflows. KAPA Pure Beads is compatible with manual processing or automated liquid handling and enables efficient recovery of input DNA in both formats.

Item #3: KAPA mRNA Hyper Prep (96rxn) (Cat. No. 08098123702)
The KAPA mRNA HyperPrep Kit is designed for both manual and automated mRNA capture and NGS library construction from 50 ng – 1000 ng of intact total RNA. The benefits of the kit are as follows:
•    Rapid and easily automatable protocol enables stranded mRNA library construction in ~5.5 hours.
•    Greater than 99% strand specificity
•    Improved coverage of GC-rich transcripts
•    Higher percentage of uniquely mapped reads
•    Streamlined "with-bead" protocol
•    Single-day library construction, inclusive of mRNA enrichment
•    KAPA Pure Beads for reaction purifications
•    Improved gene and transcript identification
•    Automation-friendly workflow
•    KAPA HiFi ensures high-efficiency and low bias amplification of GC and AT rich regions

Item #4: KAPA RNA Hyper+RiboErase HMR (96rxn) (Cat. No. 08098140702)
The KAPA RNA HyperPrep Kit with RiboErase (HMR) for Illumina sequencing contains all of the buffers and enzymes required for depletion of ribosomal RNA (rRNA) and the rapid construction of stranded RNA-Seq libraries from 25 ng – 1 µg of purified total RNA via the following steps:
•    Rapid and easily automatable protocol enables stranded mRNA library construction in ~5.5 hours.
•    Greater than 99% strand specificity
•    Improved coverage of GC-rich transcripts
•    Higher percentage of uniquely mapped reads
•    Streamlined "with-bead" protocol
•    Automation-friendly workflow
•    KAPA HiFi ensures high-efficiency and low bias amplification of GC and AT rich regions

Quantity:
One (1) x KAPA mRNA Capture Kit only (96rxn) (cat. 07962240001)
Two (2) x KAPA Pure Beads 60ml (cat. 07983298001)
Four (4) x KAPA mRNA Hyper Prep (96rxn) (cat. 08098123702)
One (1) x KAPA RNA Hyper+RiboErase HMR (96rxn) (cat. 08098140702)

Delivery Date: We require that the vendor provide these items two weeks after completing the order.