Contact Points
Lawrence, MA 01843
United States
The lab manager and end-users set up several zoom meetings with the technicians from these vendors to discuss the details of the equipment. Although come with some different specifications, all four companies were able to provide the dual injection platform for both mice and rats. And with appropriate adaptors, all of them are good for both the brain and spinal cord. However, KOPF and Stoelting cannot produce an anesthesia system (isoflurane) by themselves. KOPF adopted the same anesthesia system from WPI, and Stoelting did not respond to our request.
After we get all 4 quotes from the above-mentioned vendors, the lab manager and end-users discussed the specifications of these products. We agreed that all equipment can meet our needs and there is no significant difference in function (except for Stoelting did not response for our request for the anesthesia system). So, we compared the quotes from KOPF, RWD, and WPI, and finally picked up RWD because of obviously better price.
Our lab has routine cell cultures for different cell types for our research. It’s critical to get correct cell numbers because different cell density significantly affects cell growth and changes responses to stimulus. Currently, we are using a traditional manual count with a hemocytometer, which has a large variation in count result and largely depends on the user’s experience to tell the live/death cell by morphology.
The Acridine Orange/Propidium Iodide (AO/PI) dyes provide a highly sensitive and specific way to measure cell number and viability. The dual-fluorescence cell counter will detect live cells with AO staining (Green) and the dead cell will be recognized with PI staining (Red). This viability ratio is very important for our iPSCs to determine the quality of the culture which cannot be obtained by manual count. At the same time, the debris in the cell culture shows no fluorescence and thus is excluded from counting. Thus, we are urging to get an automated cell counter for future studies.
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