Contact Points
Statement of Need and Purpose:
The Membrane Transport Biophysics Section (MTBS) of the National Institute of Neurological Disorders and Stroke (NINDS) utilizes state-of-the-art imaging approaches to visualize and quantify membrane transporters. The MTBS Laboratory is integrating a new, high-sensitivity microscope to perform single-molecule imaging experiments. To optimize the microscope’s functionality, the MTBS requires one ultra-high-sensitivity scientific CMOS camera that is brand name or equal to the Hamamatsu ORCA-Fusion Digital CMOS Camera, which will be used in tandem with the high-sensitivity microscope to collect and digitize images for further analysis.
Background Information and Objective:
The MTBS Lab performs experiments examining the function of lysosomes from acidification and ion fluxes to cell metabolism and amino acid equilibrium. Lysosomes play a key role in cell metabolism and are implicated in numerous lysosomal storage disorders as well as broader systemic diseases. The transporters involved in these and other disorders play key roles in a variety of diseases such as cancer, osteoporosis and diabetes. An ultra-high-sensitivity camera is required to image the dynamics of these proteins at the single molecule level.
Generic Name of Product:
Ultra-high-sensitivity digital, scientific CMOS (sCMOS) camera
Brand Name or Equal to:
Product Trade Name: ORCA-Fusion Gen III sCMOS camera
Manufacturer Name: Hamamatsu
Manufacturer Model Number: C14440-20UP or C14440-20UP-KIT
Salient Characteristics / Specifications:
• Resolution: 2304 (H) x 2304 (V) pixels (5.3 megapixels)
• Speed: 100 frames/second (at 2304 x 2048 ROI)
• Dynamic range: 21,400:1
• Pixel size: 6.5μm (H) x 6.5μm (V)
• Effective area: 14.976 mm (H) ×14.976 mm (V)
• Read noise: 0.7 electrons rms
• PRNU: 0.06%
• DSNU: 0.3 electrons rms
• Peak Quantum Efficiency: 80%
• Cooling method: Peltier cooling to -5 degrees Celcius
• Lens mount: C-mount
• A/D converter: 16-bit
• Interfaces: Dual CXP-6 and USB 3.0
• Power supply: Approx. 150 VA (based on 240 V)
• USB 3.0 A/Micro-B Cable, mounting baseplate, and trigger cable included
• HCImage included
Quantity:
One (1)
Delivery Terms:
The Contractor shall deliver the above-described equipment within sixty (60) days after receipt of order to the following:
Attn: Dr. Joseph Mindell
NIH Main Campus, 35 Convent Drive
Bldg. 35A, Room 3D913
Bethesda, Maryland 20892
Delivery shall be freight on board (F.O.B.) destination, and include inside delivery. The equipment shall be delivered between the hours of 8:00am and 5:00pm local prevailing time, Monday through Friday.
Other Considerations:
The Contractor shall warrant the above-described equipment against defects in material and workmanship for a minimum of twelve (12) months from date of delivery and acceptance.
Interested parties may identify in writing their interest and capability in response to this requirement. Responses to this notice shall contain sufficient information to establish the interested parties’ bona-fide capabilities for fulfilling the requirement and include: unit price, list price, shipping and handling costs, the delivery period after contract award, the prompt payment discount terms, the F.O.B. Point (Destination or Origin), the Dun & Bradstreet Number (DUNS), the Taxpayer Identification Number (TIN), and the certification of business size. All offerors must have an active registration in the System for Award Management (SAM) www.sam.gov.
All responses must be received by closing date and must reference the announcement. Responses may be submitted electronically to the attention of the contract specialist. Fax responses will not be accepted.
All responsible sources may submit a bid, proposal, or quotation which shall be considered by the agency.