Contact Points
Microinjection into the mouse brain and electrophysiological recording of brain cells in ex vivo slices are crucial experimental approaches in the Oligodendroglial Interactions Group led by Dr. Toby Merson. The major tool used for microinjection is super fine needles pulled from glass pipettes using horizontal pipette puller. Super fine needles are also required for single cell electrophysiological recordings in brain slices. Therefore, a micropipette puller is an essential piece of equipment for the Merson Group. The P-2000 micropipette puller made by Sutter Instrument is the most trustable model for this purpose. The best laboratories worldwide including others at NIH use micropipettes generated using the P-2000/G Micropipette puller. It is essential for Dr Merson to procure this instrument for his group to ensure consistency and comparability of each microinjection manipulation.
• Color touch-screen display
• Safe heat mode to protect and extend filament life
• Pipette Cookbook program directory
• Pre-heat mode improves stability
• Line repeat mode simplifies multi-line programming
• Help topics and error detection
• Unique capacity to pull quartz glass
• Glossary with micropipette and puller terminology
• Copy & Paste function for writing new programs
• Jaw temperature sensor helps define ideal pulling conditions
• Record of last two pull results
• Ramp test more easily accessed and can now be stored and referenced within each program. Helps to establish program heat settings and protect filament.
• Two symmetrical pipettes with each pull
• Self-contained air supply with filtration system and humidity control chamber
• Memory storage for up to 100 programs
• Two cooling modes: time and delay
Microinjection into mouse brain and electrophysiological recording of brain cells in ex vivo slices is a routine experimental procedure in the Merson Group. One of the major tools used for microinjection and electrophysiological recording is the use of super fine needles pulled from glass micropipettes using horizontal pipette puller. Therefore, the micropipette puller is an essential piece of equipment for Merson Group. The P-2000/G Horizontal pipette puller made by Sutter Instrument is the most trustable tool for this purpose.
Objectives:
- To be able to produce consistent high quality microinjection needles for brain injections and electrophysiological recordings.
- To be able to use quartz glass micropipettes which offer the lowest background noise for sensitive electrophysiological experiments
Interested parties may identify in writing their interest and capability in response to this requirement. Responses to this notice shall contain sufficient information to establish the interested parties’ bona-fide capabilities for fulfilling the requirement and include: unit price, list price, shipping and handling costs, the delivery period after contract award, the prompt payment discount terms, the F.O.B. Point (Destination or Origin), the Dun & Bradstreet Number (DUNS), the Taxpayer Identification Number (TIN), and the certification of business size. All offerors must have an active registration in the System for Award Management (SAM) www.sam.gov.
All responses must be received by closing date and must reference the announcement. Responses may be submitted electronically to the attention of the contract specialist. Fax responses will not be accepted.
All responsible sources may submit a bid, proposal, or quotation which shall be considered by the agency.