POTS24-007354

Purchase Description:
(1) Chromium GEM-X Single Cell 5' Kit v3, 16 rxns (#1000699)
(2) Chromium GEM-X Single Cell 5' Chip Kit v3, 4 chips (#1000698)

. Salient characteristics
• Generate single-cell transcript counts from up to 160,000 cells per run
• Produce >180,000 individual nanoliter-scale partitions leveraging ~3.6 million unique barcodes
• Capture cells at high efficiency up to 80%
• Low doublet rate of 0.4% per 1,000 cells
• Achieve deep profiling of complex cell populations with high-throughput single cell gene expression of individual cells
• Assess the whole transcriptome with an unbiased approach appropriate for discovery
• Measure additional phenotypes, such as cell surface protein, with Feature Barcode technology
• Turn-key analysis pipelines and visualization software for novice and expert users alike
Microfluidic technology that is compatible with a wide range of eukaryotic cell sizes and types (e.g. adherent cell cultures, suspension cell cultures, tissues, blood, etc.)
• Rapid time to lysis provides an accurate view of the transcriptome with minimal impact on expression

The Chromium GEM-X Single Cell v3 assay enables single cell transcriptional profiling of up to tens of thousands of single cells. Single cell suspensions loaded onto the instrument are partitioned into GEMs, where transcripts are tagged with cell specific barcodes. The barcoded cDNA is then pooled for downstream processing and library preparation. In addition, the assay enables simultaneous assessment of gene expression and feature profiling (e.g. cell surface proteins), enabling enhanced phenotypic profiling in thousands of individual cells. The Chromium Single Cell Gene Expression assay contains reagents to construct a library using the Feature Barcode technology. However, compatible input materials, such as cell surface proteins labeled with antibody-oligonucleotide conjugates or any other products need to be prepared by the users. Precise and efficient microfluidics allow a wide dynamic range of cells to be recovered in droplets in each ~6 minute run with a low doublet rate, facilitating the profiling of precious and rare cell populations. After sequencing, downstream bioinformatics tools use the cellular barcodes to group transcripts that originated from the same cell, revealing the transcriptome and/or the feature information of each individual cell.The Chromium GEM-X Single Cell 5’ Kit v3 is used to barcode single cells as part of the 10x single cell workflow. The purpose of the kit is to yield single cell 5’-barcoded RNA for sequencing (scRNA-Seq).

GEM v3 is compatible with the Chromium GEM-X Single Cell Immune Profiling Solution is a comprehensive approach tosimultaneously examine the cellular context of the adaptive immune response and immune repertoires of hundreds to tens of thousands of T and B cells in human or mouse on a cell-by-cell basis. Single cell suspensions loaded onto the instrument are partitioned into GEMs, where transcripts are tagged with cell specific barcodes. The barcoded cDNA is then pooled for downstream processing and library preparation. The assay enables simultaneous examination of the cellular context of the adaptive immune response and immune repertoires of hundreds to tens of thousands of T and B cells in human or mouse. Further, they can be combined with the Feature Barcode technology, to detect and analyze additional cellular features, such as, cell surface proteins, CRISPR single-guide RNAs (sgRNAs) and antigen specificity to enhance immune cell phenotyping and enable the analysis of dynamic interactions between lymphocytes and target cells. Precise and efficient microfluidics allow a wide dynamic range of cells to be recovered in droplets in each ~6 minute run with a low doublet rate, facilitating the profiling of precious and rare cell populations. After sequencing, downstream bioinformatics tools use the cellular barcodes to group transcripts that originated from the same cell, revealing the transcriptome and/or the full-length T- or B-cell receptor sequences and additional cell feature information of each individual cell.



. Quantity: One each of product codes 1000699 and 1000698